Trailhead Biosystems is a developer of cell therapy using hardware and software services. The company merges developmental biology and cell therapeutics with hardware and computerized experimental design and analysis to perform process-specific parameter optimization for customers seeking to develop cell therapies. Implementing Quality by Design (QbD) principles, cell culture optimization is done by utilizing the Design of Experiment (DoE) theory combined with Multivariate Data Analysis (MVDA). This allows the company to generate mathematical models of the biological system, understand the effector-response relationship in cell culture, and provide a systems biology analysis of the differentiation space.